A dewatering plan must be submitted and approved by State and/or Regional Boards for in-water concrete use. Where possible, poured concrete should be excluded from contact with surface or groundwater during initial curing, ideally for 30 days after it is poured. During that time, runoff from the concrete will not be allowed to enter surface or groundwater. If this is not feasible due to expected flows and site conditions, commercial sealants that are non-toxic to aquatic life may be applied before it comes into contact with flowing water. Only sealants that have been tested and found nontoxic to freshwater aquatic life, including benthic macro-invertebrates, may be used on concrete surfaces that could come into contact with flowing water. Concrete is considered to be cured when water poured over the surface of concrete consistently has a pH of less than 8.5. (Note: Demonstration of nontoxicity to aquatic life may be evaluated by measuring survival of test organisms in a 96-hour bioassay. The bioassay should be performed according to the most up-to-date protocols in 40 C.F.R. part 136, currently Methods for Measuring the Acute Toxicity of Effluents and Receiving Water to Freshwater and Marine Organisms, 5th Edition (EPA-821-R-02-012), including sample collections, handling, and preservation per U.S. EPA protocols).